Table of contentsSlide 1
Definitions
Metabonomics
Slide 4
Metabonomic data
Samples for Metabonomic analysis
Slide 7
NMR VS. MS in metabonomics
Slide 9
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NMR Spectroscopy
NMR Spectroscopy of Biofluids
Slide 14
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Multivariate Statistical Analysis (Chemometrics)
Unsupervised Method - PCA (models the maximum variation in ‘X’)
Example of Principal Components Analysis (PCA)
Supervised methods (O)-PLS-DA (models the maximum separation between classes)
Summary: Typical strategy used in metabonomic analysis.
Biomarker promise…..
Slide 23
1H NMR Spectrum of NCA and TVD
Slide 25
Establishing Metabolic Signatures of Disease and Health in Humans
Major Sources of Variation in Human Populations
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Epidemiology and “OMICS”
Major problems with metabonomics
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Cancer
Past Failures and Doubts
Epithelial Ovarian Cancer
Slide 38
Chamomile Tea - Protocol
Trajectory plot of OSC-PLS model for individual subject pre and post exposure to chamomile tea.
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Conclusion
Slide 44
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Metabonomics as part of the larger picture – Integration of ‘omics’ datasets at a systems level:
Slide 47
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Acknowwledgements
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Author: M.Bitcash Homepage: ../../index.html Further information: |